論文

2024年

Heparan Sulfate Chain-Conjugated Laminin-E8 Fragments Advance Paraxial Mesodermal Differentiation Followed by High Myogenic Induction from hiPSCs
Zhao M, Taniguchi Y, Shimono C, Jonouchi T, Cheng Y, Shimizu Y, Nalbandian M, Yamamoto T, Nakagawa M, Sekiguchi K, Sakurai H*.
Adv Sci (Weinh). 2024 Jul;11(26):e2308306. doi: 10.1002/advs.202308306. Epub 2024 Apr 29.

Possible involvement of zinc transporter ZIP13 in myogenic differentiation
Shoji M, Ohashi T, Nagase S, Yuri H, Ichihashi K, Takagishi T, Nagata Y, Nomura Y, Fukunaka A, Kenjou S, Miyake H, Hara T, Yoshigai E, Fujitani Y, Sakurai H, Dos Santos HG, Fukada T, Kuzuhara T.
Sci Rep. 2024 Apr 12;14(1):8052. doi: 10.1038/s41598-024-56912-7.

iMSC-mediated delivery of ACVR2B-Fc fusion protein reduces heterotopic ossification in a mouse model of fibrodysplasia ossificans progressiva
Gao P, Inada Y, Hotta A, Sakurai H, Ikeya M.
Stem Cell Res Ther. 2024 Mar 18;15(1):83. doi: 10.1186/s13287-024-03691-7.

Cell Therapy for Duchenne Muscular Dystrophy Using Induced Pluripotent Stem Cell-derived Muscle Stem Cells and the Potential of Regenerative Rehabilitation
Takenaka-Ninagawa N, Goto M, Yoshioka CKB, Miki M, Sakurai H*
Current Opinion in Biomedical Engineering, 2024 Feb 30:100523

2023年

Uniform transgene activation in Tet-On systems depends on sustained rtTA expression.
Otomo J, Woltjen K, Sakurai H*.
iScience. 2023 Aug 19;26(10):107685. doi: 10.1016/j.isci.2023.107685. eCollection 2023 Oct 20.

Dual CRISPR-Cas3 system for inducing multi-exon skipping in DMD patient-derived iPSCs.
Kita Y, Okuzaki Y, Naoe Y, Lee J, Bang U, Okawa N, Ichiki A, Jonouchi T, Sakurai H, Kojima Y, Hotta A.
Stem Cell Reports. 2023 Sep 12;18(9):1753-1765. doi: 10.1016/j.stemcr.2023.07.007. Epub 2023 Aug 24.

Simple and efficient differentiation of human iPSCs into contractible skeletal muscles for muscular disease modeling.
Rashid MI, Ito T, Miya F, Shimojo D, Arimoto K, Onodera K, Okada R, Nagashima T, Yamamoto K, Khatun Z, Shimul RI, Niwa JI, Katsuno M, Sobue G, Okano H, Sakurai H, Shimizu K, Doyu M, Okada Y.
Sci Rep. 2023 May 25;13(1):8146. doi: 10.1038/s41598-023-34445-9.

Screening Station, a novel laboratory automation system for physiologically relevant cell-based assays.
Namatame I, Ishii K, Shin T, Shimojo D, Yamagishi Y, Asano H, Kishimoto Y, Fuse H, Nishi Y, Sakurai H, Nakahata T, Sasaki-Iwaoka H.
SLAS Technol. 2023 Oct;28(5):351-360. doi: 10.1016/j.slast.2023.04.002. Epub 2023 Apr 28.

A new immunodeficient Duchenne muscular dystrophy rat model to evaluate engraftment after human cell transplantation.
Sato M, Goto M, Yamanouchi K, Sakurai H*.
Front Physiol. 2023 Apr 10;14:1094359. doi: 10.3389/fphys.2023.1094359. eCollection 2023.

A mutation in DOK7 in congenital myasthenic syndrome forms aggresome in cultured cells, and reduces DOK7 expression and MuSK phosphorylation in patient-derived iPS cells.
Zhang S, Ohkawara B, Ito M, Huang Z, Zhao F, Nakata T, Takeuchi T, Sakurai H, Komaki H, Kamon M, Araki T, Ohno K.
Hum Mol Genet. Advance access publication date 29 December 2022, 2023 Apr 20;32(9):1511-1523. doi: 10.1093/hmg/ddac306.

Evaluation of Human-Induced Pluripotent Stem Cells Derived from a Patient with Schwartz-Jampel Syndrome Revealed Distinct Hyperexcitability in the Skeletal Muscles.
Yamashita Y, Nakada S, Nakamura K, Sakurai H, Ohno K, Goto T, Mabuchi Y, Akazawa C, Hattori N, Arikawa-Hirasawa E.
Biomedicines. 2023 Mar 7;11(3):814. doi: 10.3390/biomedicines11030814.

Establishment of quantitative and consistent in vitro skeletal muscle pathological models of myotonic dystrophy type 1 using patient-derived iPSCs.
Kawada R, Jonouchi T, Kagita A, Sato M, Hotta A, Sakurai H*.
Sci Rep. 2023 Jan 11;13(1):94. doi: 10.1038/s41598-022-26614-z

2022年

Induction of functional xeno-free MSCs from human iPSCs via a neural crest cell lineage
Kamiya D, Takenaka-Ninagawa N, Motoike S, Kajiya M, Akaboshi T, Zhao C, Shibata M, Senda S, Toyooka Y, Sakurai H, Kurihara H, Ikeya M.
NPJ Regen Med. 2022 Sep 15;7(1):47. doi: 10.1038/s41536-022-00241-8.

Pathophysiological levels of GDF11 activate Smad2/Smad3 signaling and induce muscle atrophy in human iPSC-derived myocytes
Honda M, Makino T, Zhao X, Matsuto M, Sakurai H, Takahashi Y, Shimizu M, Sato R, Yamauchi Y.
Am J Physiol Cell Physiol. 2022 Nov 1;323(5):C1402-C1409. doi: 10.1152/ajpcell.00341.2022. Epub 2022 Sep 12.
Skeletal muscle releases extracellular vesicles with distinct protein and microRNA signatures that function in the muscle microenvironment.
Watanabe S, Sudo Y, Makino T, Kimura S, Tomita K, Noguchi M, Sakurai H, Shimizu M, Takahashi Y, Sato R, Yamauchi Y.
PNAS Nexus. 2022 Aug 26;1(4):pgac173. doi: 10.1093/pnasnexus/pgac173. eCollection 2022 Sep

Dissociation of SH3 and cysteine rich domain 3 and junctophilin 1 from dihydropyridine receptor in dystrophin-deficient muscles.
Ashida Y, Himori K, Tokuda N, Naito A, Yamauchi N, Takenaka-Ninagawa N, Aoki Y, Sakurai H, Yamada T.
Am J Physiol Cell Physiol. 2022 Sep 1;323(3):C885-C895. doi: 10.1152/ajpcell.00163.2022. Epub 2022 Aug 1.

Cell type-specific abnormalities of central nervous system in myotonic dystrophy type 1.
Nakamori M, Shimizu H, Ogawa K, Hasuike Y, Nakajima T, Sakurai H, Araki T, Okada Y, Kakita A, Mochizuki H.
Brain Commun. 2022 Jun 10;4(3):fcac154. doi: 10.1093/braincomms/fcac154. eCollection 2022.

Mature Myotubes Generated From Human-Induced Pluripotent Stem Cells Without Forced Gene Expression.
Fujiwara K, Yamamoto R, Kubota T, Tazumi A, Sabuta T, Takahashi MP, Sakurai H.
Front Cell Dev Biol. 2022 May 30;10:886879. doi: 10.3389/fcell.2022.886879. eCollection 2022.

Single-cell RNA-seq reveals heterogeneity in hiPSC-derived muscle progenitors and E2F family as a key regulator of proliferation.
Nalbandian M, Zhao M, Kato H, Jonouchi T, Nakajima-Koyama M, Yamamoto T, Sakurai H.
Life Sci Alliance. 2022 Apr 22;5(8):e202101312. doi: 10.26508/lsa.202101312. Print 2022 Aug

Transplantation of human iPSC-derived muscle stem cells in the diaphragm of Duchenne muscular dystrophy model mice.
Miura Y, Sato M, Kuwahara T, Ebata T, Tabata Y, Sakurai H.
PLoS One. 2022 Apr 4;17(4):e0266391. doi: 10.1371/journal.pone.0266391. eCollection 2022.

2021年

Systemic Supplementation of Collagen VI by Neonatal Transplantation of iPSC-Derived MSCs Improves Histological Phenotype and Function of Col6-Deficient Model Mice.
Harada A, Goto M, Kato A, Takenaka-Ninagawa N, Tanaka A, Noguchi S, Ikeya M, Sakurai H.
Front Cell Dev Biol. 2021 Nov 23;9:790341. doi: 10.3389/fcell.2021.790341. eCollection 2021.

Orai1-STIM1 Regulates Increased Ca2+ Mobilization, Leading to Contractile Duchenne Muscular Dystrophy Phenotypes in Patient-Derived Induced Pluripotent Stem Cells.
Uchimura T, Sakurai H.
Biomedicines. 2021 Oct 31;9(11):1589. doi: 10.3390/biomedicines9111589

Contractile Activity of Myotubes Derived from Human Induced Pluripotent Stem Cells: A Model of Duchenne Muscular Dystrophy.
Yoshioka K, Ito A, Horie M, Ikeda K, Kataoka S, Sato K, Yoshigai T, Sakurai H, Hotta A, Kawabe Y, Kamihira M.
Cells. 2021 Sep 27;10(10):2556. doi: 10.3390/cells10102556.

Restoration of the defect in radial glial fiber migration and cortical plate organization in a brain organoid model of Fukuyama muscular dystrophy.
Taniguchi-Ikeda M, Koyanagi-Aoi M, Maruyama T, Takaori T, Hosoya A, Tezuka H, Nagase S, Ishihara T, Kadoshima T, Muguruma K, Ishigaki K, Sakurai H, Mizoguchi A, Novitch BG, Toda T, Watanabe M, Aoi T.
iScience. 2021 Sep 17;24(10):103140. doi: 10.1016/j.isci.2021.103140. eCollection 2021 Oct 22

Collagen-VI supplementation by cell transplantation improves muscle regeneration in Ullrich congenital muscular dystrophy model mice.
Takenaka-Ninagawa N, Kim J, Zhao M, Sato M, Jonouchi T, Goto M, Yoshioka CKB, Ikeda R, Harada A, Sato T, Ikeya M, Uezumi A, Nakatani M, Noguchi S, Sakurai H.
Stem Cell Res Ther. 2021 Aug 9;12(1):446. doi: 10.1186/s13287-021-02514-3.

A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs
Uchimura T, Asano T, Nakata T, Hotta A, Sakurai H
Cell Rep Med. 2021 Jun 4;2(6):100298. doi: 10.1016/j.xcrm.2021.100298.

Characterization of hiPSC-derived muscle progenitors reveals distinctive markers for myogenic cell purification toward cell therapy
Nalbandian M, Zhao M, Sasaki-Honda M, Jonouchi T, Lucena-Cacace A, Mizusawa T, Yasuda M, Yoshida Y, Hotta A, Sakurai H
Stem Cell Reports. 2021 Apr 13;16(4):883-898. doi: 10.1016/j.stemcr.2021.03.004.

Establishment of a Robust Platform for Induced Pluripotent Stem Cell Research Using Maholo LabDroid
Sasamata M, Shimojo D, Fuse H, Nishi Y, Sakurai H, Nakahata T, Yamagishi Y, Sasaki-Iwaoka H
SLAS Technology, Mar 27:24726303211000690. doi: 10.1177/24726303211000690. Online ahead of print.

Nanopore direct RNA sequencing detects DUX4-activated repeats and isoforms in human muscle cells.
Mitsuhashi S, Nakagawa S, Sasaki-Honda M, Sakurai H, Frith MC, Mitsuhashi H.
Hum Mol Genet. 2021 Mar 9:ddab063. doi: 10.1093/hmg/ddab063. Online ahead of print

MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells.
Iwasaki H, Ichihara Y, Morino K, Lemecha M, Sugawara L, Sawano T, Miake J, Sakurai H, Nishi E, Maegawa H, Imamura T.
Sci Rep. 2021 Jan 13;11(1):1161. doi: 10.1038/s41598-020-80742-y.

2020年

Generation of a transgene-free iPSC line and genetically modified line from a facioscapulohumeral muscular dystrophy type 2 (FSHD2) patient with SMCHD1 p.Lys607Ter mutation.
Sasaki-Honda M, Kagita A, Jonouchi T, Araki T, Hotta A, Sakurai H.
Stem Cell Res. 2020 Jun 24;47:101884. doi: 10.1016/j.scr.2020.101884. Online ahead of print

Induced Fetal Human Muscle Stem Cells with High Therapeutic Potential in a Mouse Muscular Dystrophy Model.
Zhao M, Tazumi A, Takayama S, Takenaka-Ninagawa N, Nalbandian M, Nagai M, Nakamura Y, Nakasa M, Watanabe A, Ikeya M, Hotta A, Ito Y, Sato T, Sakurai H.
Stem Cell Reports. 2020 Jun 23: S2213-6711(20)30225-3. doi: 10.1016/j.stemcr.2020.06.004. Online ahead of print.

Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping.
Gee P, Lung MSY, Okuzaki Y, Sasakawa N, Iguchi T, Makita Y, Hozumi H, Miura Y, Yang LF, Iwasaki M, Wang XH, Waller MA, Shirai N, Abe YO, Fujita Y, Watanabe K, Kagita A, Iwabuchi KA, Yasuda M, Xu H, Noda T, Komano J, Sakurai H, Inukai N, Hotta A.
Nat Commun. 2020 Mar 13;11(1):1334. doi: 10.1038/s41467-020-14957-y

2019年

A Liver Model of Infantile-Onset Pompe Disease Using Patient-Specific Induced Pluripotent Stem Cells.
Yoshida T, Jonouchi T, Osafune K, Takita J, Sakurai H.
Front Cell Dev Biol. 2019 Nov 29;7:316. doi: 10.3389/fcell.2019.00316. eCollection 2019.

Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations.
Grajcarek J, Monlong J, Nishinaka-Arai Y, Nakamura M, Nagai M, Matsuo S, Lougheed D, Sakurai H, Saito MK, Bourque G, Woltjen K.
Nat Commun. 2019 Oct 24;10(1):4856. doi: 10.1038/s41467-019-12829-8

Identification of 2,6-Disubstituted 3H-Imidazo[4,5-b]pyridines as Therapeutic Agents for Dysferlinopathies through Phenotypic Screening on Patient-Derived Induced Pluripotent Stem Cells.
Takada H, Kaieda A, Tawada M, Nagino T, Sasa K, Oikawa T, Oki A, Sameshima T, Miyamoto K, Miyamoto M, Kokubu Y, Tozawa R, Sakurai H, Saito B.
J Med Chem. 2019 Oct 24;62(20):9175-9187. doi: 10.1021/acs.jmedchem.9b01100. Epub 2019 Oct 7.

iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases.
Lin CY, Yoshida M, Li LT, Ikenaka A, Oshima S, Nakagawa K, Sakurai H, Matsui E, Nakahata T, Saito MK.
JCI Insight. 2019 Sep 19;4(18). pii: 124299. doi: 10.1172/jci.insight.124299.

Core Transcription Factors Promote Induction of PAX3-Positive Skeletal Muscle Stem Cells.
Sato T, Higashioka K, Sakurai H, Yamamoto T, Goshima N, Ueno M, Sotozono C.
Stem Cell Reports. 2019 Aug 13;13(2):352-365. doi: 10.1016/j.stemcr.2019.06.006. Epub 2019 Jul 25

Human Skeletal Muscle Cells Derived from the Orbicularis Oculi Have Regenerative Capacity for Duchenne Muscular Dystrophy.
Yamanaka Y, Takenaka N, Sakurai H, Ueno M, Kinoshita S, Sotozono C, Sato T.
Int J Mol Sci. 2019 Jul 14;20(14). pii: E3456. doi: 10.3390/ijms20143456.

RNA Virus-Based Episomal Vector with a Fail-Safe Switch Facilitating Efficient Genetic Modification and Differentiation of iPSCs.
Komatsu Y, Takeuchi D, Tokunaga T, Sakurai H, Makino A, Honda T, Ikeda Y, Tomonaga K.
Mol Ther Methods Clin Dev. 2019 May 28;14:47-55. doi: 10.1016/j.omtm.2019.05.010. eCollection 2019 Sep 13

Phenotypic drug screening for dysferlinopathy using patient-derived induced pluripotent stem cells.
Kokubu Y, Nagino T, Sasa K, Oikawa T, Miyake K, Kume A, Fukuda M, Fuse H, Tozawa R, Sakurai H.
Stem Cells Transl Med. 2019 Oct;8(10):1017-1029. doi: 10.1002/sctm.18-0280. Epub 2019 Jun 28.

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells.
Nakajima T, Sakurai H, Ikeya M.
J Vis Exp. 2019 Apr 25;(146). doi: 10.3791/59359

2018年

Identification of Novel Antisense-Mediated Exon Skipping Targets in DYSF for Therapeutic Treatment of Dysferlinopathy.
Lee JJA, Maruyama R, Duddy W, Sakurai H, Yokota T.
Mol Ther Nucleic Acids. 2018 Dec 7;13:596-604. doi: 10.1016/j.omtn.2018.10.004. Epub 2018 Oct 11.

Modeling human somite development and fibrodysplasia ossificans progressiva with induced pluripotent stem cells.
Nakajima T, Shibata M, Nishio M, Nagata S, Alev C, Sakurai H, Toguchida J, Ikeya M.
Development. 2018 Aug 23;145(16). pii: dev165431. doi: 10.1242/dev.165431

A Patient-derived iPSC Model Revealed Oxidative Stress Increases Facioscapulohumeral Muscular Dystrophy-causative DUX4.
Sasaki-Honda M, Jonouchi T, Arai M, Hotta A, Mitsuhashi S, Nishino I, Matsuda R, Sakurai H.
Hum Mol Genet. 2018 Dec 1;27(23):4024-4035. doi: 10.1093/hmg/ddy293.

Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro.
Ishii K, Sakurai H, Suzuki N, Mabuchi Y, Sekiya I, Sekiguchi K, Akazawa C.
Stem Cell Reports. 2018 Jan 10. pii: S2213-6711(17)30561-1.
doi: 10.1016/j.stemcr.2017.12.013.

Cell Membrane Repair Assay Using a Two-photon Laser Microscope.
Lee JJA, Maruyama R, Sakurai H, Yokota T.
J Vis Exp. 2018 Jan 2;(131). doi: 10.3791/56999.

2017年

A human iPS cell myogenic differentiation system permitting high-throughput drug screening.
Uchimura T, Otomo J, Sato M, Sakurai H.
Stem Cell Res. 2017 Dec;25:98-106. doi: 10.1016/j.scr.2017.10.023. Epub 2017 Oct 28

A Skeletal Muscle Model of Infantile-onset Pompe Disease with Patient-specific iPS Cells.
Yoshida T, Awaya T, Jonouchi T, Kimura R, Kimura S, Era T, Heike T, Sakurai H.
Scientific Reports. 2017 Oct 18;7(1):13473. doi: 10.1038/s41598-017-14063-y.

Myogenic Differentiation from MYOGENIN-Mutated Human iPS Cells by CRISPR/Cas9.
Higashioka K, Koizumi N, Sakurai H, Sotozono C, Sato T.
Stem Cells Int. 2017;2017:9210494.

Myotonic dystrophy type 1 patient-derived iPSCs for the investigation of CTG repeat instability
Ueki J, Nakamori M, Nakamura M, Nishikawa M, Yoshida Y, Tanaka A, Morizane A, Kamon M, Araki T, Takahashi M.P., Watanabe A, Inagaki N, Sakurai H.
Scientific Reports. 2017 Feb 13;7:42522.

2016年

Functional validation and expression analysis of myotubes converted from skin fibroblasts using a simple direct reprogramming strategy.
Horio F, Sakurai H, Ohsawa Y, Nakano S, Matsukura M, Fujii I.
eNeurologicalSci. 2016 Nov 3;6:9-15. doi: 10.1016/j.ensci.2016.11.002. eCollection 2017 Mar. PMID: 29260008

2015年

MicroRNA-494 plays a role in fiber type-specific skeletal myogenesis in human induced pluripotent stem cells.
Iwasaki H, Imamura T, Morino K, Shimosato T, Tawa M, Ugi S, Sakurai H, Maegawa H, Okamura T.
Biochem Biophys Res Commun. 2015 Oct 28. pii: S0006-291X(15)30827-5. doi: 10.1016/j.bbrc.2015.10.128.

Early pathogenesis of Duchenne muscular dystrophy modelled in patient-derived human induced pluripotent stem cells.
Shoji E., Sakurai H., Nishino T., Nakahata T., Heike T., Awaya T., Fujii N., Manabe Y., Matsuo M., Sehara-Fujisawa A.
Scientific Reports. 2015 :5, Article number: 12831; doi:10.1038/srep12831

Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9.
Li HL., Fujimoto N., Sasakawa N., Shirai S., Ohkame T., Sakuma T., Tanaka M., Amano N., Watanabe A., Sakurai H., Yamamoto T., Yamanaka S., Hotta A.
Stem Cell Reports. 2015 Jan 13;4(1):143-54.

2014年

Functional analysis of iPSC-derived myocytes from a patient with carnitine palmitoyltransferase II deficiency.
Yasuno T., Osafune K., Sakurai H., Asaka I., Tanaka A., Yamaguchi S., Yamada K., Hitomi H., Arai S., Kurose Y., Higaki Y., Sudo M., Ando S., Nakashima H., Saito T., Kaneoka H.
Biochem Biophys Res Commun. 2014 May 30;448(2):175-81.

2013年

In vitro characterization and engraftment of adipocytes derived from human induced pluripotent stem cells and embryonic stem cells.
Noguchi M., Hosoda K., Nakane M., Mori E., Nakao K., Taura D., Yamamoto Y., Kusakabe T., Sone M., Sakurai H., Fujikura J., Ebihara K., Nakao K..
Stem Cells Dev. 2013 Nov 1;22(21):2895-905.

Fetal skeletal muscle progenitors have regenerative capacity after intramuscular engraftment in dystrophin deficient mice.
Sakai H., Sato T., Sakurai H., Yamamoto T., Hanaoka K., Montarras D., Sehara-Fujisawa A.
PLoS One. 2013 May 9;8(5):e63016

Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Myoshi Myopathy in vitro
Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H.
PLoS One. 2013, 8(4):e61540

2012年

In Vitro Modeling of Paraxial Mesodermal Progenitors Derived from Induced Pluripotent Stem Cells
Sakurai H., Sakaguchi Y., Shoji E., Nishino T., Maki I., Sakai H., Hanaoka K., Kakizuka A., Sehara-Fujisawa A
PLoS ONE. 2012,7(10): e47078.

2011年

Differentiation of induced pluripotent stem cells to thymic epithelial cells by phenotype
Inami Y., Yoshikai T., Ito S., Nishio N., Suzuki H., Sakurai H., Isobe K.
Immunology and Cell Biology. 2011, 89, 314-21.

2010年

GADD34 suppresses wound healing by upregulating expression of myosin IIA
Tanaka C, Ito S, Nishio N, Kodera Y, Sakurai H, Suzuki H, Nakao A, Isobe K.
Transgenic Res. 2010 Aug;19(4):637-45.

Metalloprotease-Dependent Onset of Blood Circulation in Zebrafish
Iida A., Sakaguchi K., Sato K., Sakurai H., Nishimura D., Iwaki A., Takeuchi M., Kobayashi M., Misaki K., Yonemura S., Kawahara A., Sehara-Fujisawa A.
Current Biology. 2010 June; 20(12): 1110-16.

2009年以前

Bidirectional induction toward paraxial mesodermal derivatives from mouse ES cells in chemically defined medium
Sakurai H., Inami Y., Tamamura Y., Yoshikai T., Sehara-Fujisawa A., Isobe K.
Stem Cell Research. 2009 Sep-Nov;(2-3): 157-69.

Paraxial mesodermal progenitors derived from mouse embryonic stem cells contribute to muscle regeneration via differentiation into muscle satellite cells
Sakurai H., Okawa Y., Inami Y., Nishio N., Isobe K.
Stem Cells. 2008 Jul;26(7): 1865-73

Neutrophil depletion delays wound repair in aged mice
Nishio N., Okawa Y., Sakurai H., Isobe K.
Age. 2008; 30: 11-19

In vitro modeling of paraxial and lateral mesoderm differentiation reveals early reversibility
Sakurai H., Era T., Lakt LM., Okada M., Nakai S., Nishikawa S., Nishikawa SI.
Stem Cells. 2006 Mar;24(3): 575-86

Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture
Tada S., Era T., Furusawa C., Sakurai H., Nishikawa S., Kinoshita M., Chiba T., Nishikawa SI.
Development. 2005 Oct;132(19): 4363-74

プロトコール

In Vitro Evaluation of Exon Skipping in Disease-Specific iPSC-Derived Myocytes.
Zhao M, Shoji E, Sakurai H.
Methods Mol Biol. 2018;1828:173-189. doi: 10.1007/978-1-4939-8651-4_11

Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells.
Shoji E, Woltjen K, Sakurai H.
Methods in Mol Biol. 2015 May 14.

ヒトiPS細胞から骨格筋細胞への効率的な分化誘導
庄子栄美、櫻井英俊
ES・iPS細胞実験スタンダード(羊土社) 2014:194-201

著作物

1.「多能性幹細胞による筋ジストロフィー治療」
櫻井英俊
生体の科学(医学書院) 62(2), 161-164, 2011

日本語解説

1. iPS細胞を使った筋疾患研究
櫻井英俊
病理と臨床 2015:33(6): 592-598

2. 患者由来iPS細胞による筋疾患モデル作製
櫻井英俊
医学のあゆみ 2015:252(9): 969-975

3. 三好型ミオパチー
櫻井英俊
遺伝子医学MOOK 2015:“27 iPS細胞を用いた難病研究”: 71-76

4. iPS細胞を用いた難治性筋疾患治療研究
櫻井英俊
実験医学2014:32(9):1359-1365

5. iPS細胞創薬は10年以内に実現するか?「Yes」の立場から
櫻井英俊
Frontiers in Parkinson Disease 2014:7(4):200-203

6. iPS細胞を用いた筋疾患モデル
櫻井英俊
最新医学 2014:69: 589-597

7. iPS細胞技術を用いた筋再生療法の開発
櫻井英俊
整形・災害外科 2013:56(5):689-694

8. iPS細胞を用いた筋ジストロフィー症の病態解析
竹中菜々、櫻井英俊
Medical Science Digest 2013:39(11): 514-517

9. iPS細胞を使う―骨格筋の研究へ―
竹中菜々、櫻井英俊
整形外科 2013:64(11): 1221-1223

10. iPS細胞
櫻井英俊、山中伸弥
先進医療 NAVIGATOR 2013: 93-95

11. 多能性幹細胞による筋ジストロフィー治療
櫻井英俊
生体の科学(医学書院)  2011:62(2): 161-164

12. iPS細胞(人工多能性幹細胞)研究
櫻井英俊、山中伸弥
日本臨床 2011:69(12): 2114-18